English Name：GUO Yongfeng

中文名： 郭永峰

Phone：+8653266715256

Fax：+865328870205

Email：guoyongfeng@caas.cn

Academic Title：Professor

Supervisor：Master Supervisor, PhD Supervisor

Department：Biotechnology Research Center

Group：Tobacco Functional Genomics

Research Area：Plant peptide hormones, Leaf senescence, Plant molecular farming

Publications：

Wu Y^#, Ni Q^#, Akher SA, Zhang Z, Wang J*, Guo Y*, Zhang J* (2026) Improving heterologous

protein production by modulating ROS homeostasis in Nicotiana benthamiana. Molecular Horticulture (In press).

Diao HP^#, Meng HX^#, Xu XJ, Zhang ZL, Zhang JF, Guo YF*, I Hwang*, Song SJ* (2026)

Knocking out two polyphenol oxidase genes significantly improves recombinant protein purification in Nicotiana benthamiana. Plant Biotechnology Journal  https://doi.org/10.1111/pbi.70575

Li Y^#, Liu T^#, Zhu L^#, Wu R, Liu Y, Zhou H, Guo W, Zhang S, Akher SA, Piao H, Zhang J,

Liang K, Zhao Z, Guo Y, Jin Z*, Zhang Z* (2026) Analysis of the aspartic protease gene family in Nicotiana benthamiana and its application in recombinant protein expression. Frontiers in Plant Science 17:1778448.

Wang, K, Guo Y, eds. (2026) Plant molecular farming for biopharmaceutical production and

beyond. Lausanne: Frontiers Media SA. Switzerland, 179 pages. doi: 10.3389/978-2-

8325-7363-1

Wang KY*, Guo Y* (2026) Editorial: plant molecular farming for biopharmaceutical production

and beyond. Frontiers in Plant Science 16:1765245.

Diao HP, Guo YF*, Chen Y*, Hwang I*, Song SJ* (2026) Plant-derived vaccines: advances in

delivery approaches. Plant Communications 7: 101676.

Meng HX^#, Diao HP^#, Sun JH, Ren SY, Xu XJ, Guo YF, Song SJ*. (2026) SNAC-Tag

integration into NPN platform facilitates efficient purification of recombinant proteins from Nicotiana benthamiana. Plant Biotechnology Journal 24:87-89.

Liu T^#, Kong N^#, Cao J, Chen G, Deng Z, Wen L, Zhang Z, Guo C, Wu R, Yang Y, Li W*, Guo

Y* (2025) Transcriptomic and metabolomic analysis of carotenoid metabolism and accumulation of carotenoid-derived products in tobacco leaves before and after curing. Frontiers in Plant Science 16: 1671379.

Meng HX^#, Ren SY^#, Xu XJ, Guo YF, Diao HP, Song SJ. (2025) Protocol for preparing natural

peptidoglycan nanoparticles and purifying plant-derived antigens. STAR Protocols 6(4):104260.

Li X, Si X, Zhang Z, Ni Q, Li W, Zhang Z, Wang J*, Guo Y* (2025) Peptide signals regulating

leaf senescence. Annals of Botany mcaf140.

Song SJ^#*, Diao HP^#, Zhang SS, Kang S, Liu C, Kim S, Yun J, Meng HX, Moon H, Kim WY,

Kim KH, Yang MJ, Kim KS, Guo YF, Hwang I* (2025) Natural peptidoglycan nanoparticles enable rapid antigen purification and potent delivery for plant-derived vaccine. Plant Communications 6:101418.

Yang Y^#, Deng Z^#, Zhang Z, Yang Y, Pan X,  WuR, Liu T, Gao X, Li L*, Guo Y* (2025)  

Papain-like cysteine proteases in Nicotiana benthamiana: gene family members  and their potential implications in  recombinant protein expression.  Frontiers in Plant Science 16:1565487.

Akher SA^#, Wang KY^#, Hall K, Hunpatin OS, Shan M, Zhang Z*, Guo Y* (2025)

Harnessing transient expression systems with plant viral vectors for the production of biopharmaceuticals in Nicotiana benthamiana. International Journal of Molecular Science 26:5510.

Deng Z^#, Pan X^#, Wu R, Yang Y, Liu T, Li W, Zhang Z, Guo Y* (2025) Tobacco (Nicotiana

tabacum) PSY peptides and their potential roles in seed germination, vegetative growth, and leaf senescence under osmotic stress. Frontiers in Plant Science 16:1575308.

Deng Z^#, Yang Y^#, Marowa P, Pan X, Wu R, Liu T, Guo Y* (2025) Processing of plant peptide

signals: critical steps mediated by subtilisins. Plant Hormones 1: e003.

Zhang Z^#, Han H^#, Zhao J^#, Liu Z^#, Deng L^#, Wu L^#, Niu J, Guo Y*, Wang G*, Gou X*, Li C*,

Li C-Y* and Liu C-M* (2025) Peptide hormones in plants. Molecular Horticulture 5:7.

Zhang Z, Gigli-Bisceglia N*, Li W, Li S, Wang J, Liu J, Testerink C, Guo Y* (2024)  

SCOOP10 and SCOOP12 peptides act through MIK2 receptor-like kinase to antagonistically regulate Arabidopsis leaf senescence. Molecular Plant 17:1805-1819.

Zhang P, Zhao J, Zhang W, Guo Y, Zhang K (2024) Sulfated peptides: key players in plant

development, growth, and stress responses. Frontiers in Plant Science 15:1474111.

Zhang Z^#, Shan M^#, Yang L^#, Cao S, Wang J, Li W*, Guo Y* (2024) Identification of

transcription factors associated with leaf senescence in tobacco. Scientific Reports 14(1):21556.

Song SJ, Diao HP, Guo YF, Hwang I* (2024) Advances in subcellular accumulation design for

recombinant protein production in tobacco. BioDesign Research 6:0047.

Xie M^#, Ding A^#, Guo Y, Sun J, Qiu W, Chen M, Li Z, Li S, Zhou G, Xu Y, Wang M, Richel A*,

Gong D*, Kong Y* (2024) The transcription factors ZAT5 and BLH2/4 regulate homogalacturonan demethylesterification in Arabidopsis seed coat mucilage. Plant Cell koae209.

Pan X^#, Deng Z^#, Wu R, Yang Y, Akher SA, Li W, Zhang Z*, Guo Y* (2024) Identification of

CEP peptides encoded by the tobacco (Nicotiana tabacum) genome and characterization of their roles in osmotic and salt stress responses. Plant Physiology and Biochemistry 209:108525.

Zhang Z, Zhang Z, Akher SA, Xue J, Wang J, Guo C, Li Z*, Guo Y* (2024) Integrative analysis

of the DUF668 gene family in Nicotiana tabacum to excavate their potential roles in abiotic stress responses. Agronomy 14, 445.

Wu R^#, Pan X^#, Li W, Zhang Z*, Guo Y* (2024) Phosphorylation of Thr-225 and Ser-262 on

ERD7 promotes age-dependent and stress-induced leaf senescence through the regulation of hydrogen peroxide accumulation in Arabidopsis thaliana. International Journal of

Molecular Sciences 25, 1328.

Zhang Z, Zhang Z, Shan M, Amjad Z, Xue J, Zhang Z, Wang J, Guo Y* (2024) Genome-wide

studies of FH family members in soybean ( Glycine max) and their responses under abiotic stresses. Plants 13(2):276.

Lu M, Fu B, Meng X, Jia T, Lu X, Yang C, Li K, Yin P, Guo Y, Li W, Chi J, Wang G*, Zhou

C* (2024) NtNAC028 and NtNAC080 form heterodimers to regulate jasmonic acid biosynthesis during leaf senescence in Nicotiana tabacum. Journal of Experimental Botany 75(8): 2351-2371.

Xu K^#, Tian D^#, Wang T, Zhang A, Elsadek MAY, Liu W, Chen L* & Guo Y* (2023) Small

secreted peptides (SSPs) in tomato and their potential roles in drought stress response. Molecular Horticulture 3, 17.

Wang Q, Li X, Guo C, Wen L, Deng Z, Zhang Z, Li W, Liu T, Guo Y* (2023) Senescence-

Related Receptor Kinase 1 (SENRK1) functions downstream of WRKY53 in regulating leaf senescence in Arabidopsis. Journal of Experimental Botany 74(17):5140-5152.

Wen L^#, Cao J ^#, Li W*, Guo Y* (2023) Changes in volatile profile and related gene expression

during senescence of tobacco leaves. Journal of the Science of Food and Agriculture Doi:10.1002/jsfa.12733

Chu Y^#, Gao X^#, Wen L, Deng Z, Liu T, Guo Y* (2023) Characterization of the CLE Family in

three Nicotiana species and potential roles of CLE peptides in osmotic and salt stress responses. Agronomy 13:1480.

Tian D, Xie Q, Deng Z, Xue J, Li W, Zhang Z, Dai Y, Zheng B, Lu T, De Smet I, Guo Y* (2022)

Small secreted peptides encoded on the wheat (Triticum aestivum L.) genome and their

potential roles in stress responses. Frontiers in Plant Science 13:1000297.

Wen L, Liu T, Deng Z, Zhang Z, Wang Q, Wang W, Li W*, Guo Y* (2022) Characterization of

NAC transcription factor NtNAC028 as a regulator of leaf senescence and stress

responses. Frontiers in Plant Science 13:941026.

Guo C^#, Li X^#, Zhang Z, Wang Q, Zhang Z, Wen L, Liu C, Deng Z, Chu Y, Liu T, Guo Y* 

(2022) The INFLORESCENCE DEFICIENT IN ABSCISSION-LIKE6 peptide functions

as a positive modulator of leaf senescence in Arabidopsis thaliana.  Frontiers in Plant

Science 13: 909378.

Li X, Guo C, Wang Q, Li Z, Cai J, Wu D, Li Y, Yang A, Guo Y, Gao J, Wen L* and Pu W*

(2022) Systematic Analysis of Tobacco CrRLK1L Family genes and functional

identification of NtCrRLK1L47 in environmental stresses. Frontiers in Plant Science

13:838857.

Liu X, Chen X, Zhu X, Lin Q, Pan X, Tan X, Guo Y, Qiu J* and Fang S* (2022) Establishment

and comparative analysis of enzyme-linked immunoassay and time-resolved fluoroimmunoassay for the determination of trace quinclorac in environment. Biosensors 12(5), 338.

Li X, Wang Q, Guo C, Sun J, Li Z, Wang Y, Yang A, Pu W, Guo Y, Gao J* and Wen L* (2022)

NtNAC053, a novel NAC transcription factor, confers drought and salt tolerances in

tobacco. Frontiers in Plant Science 13:817106.

Guo Y, Balazadeh S, Paek N-C, eds. (2022) Signaling Events in Regulating Leaf Senescence.

Lausanne: Frontiers Media SA, Switzerland, 134 pages. doi: 10.3389/978-2-88974-687-3.

Guo Y*, Balazadeh S, Paek NC (2022) Editorial: signaling events in regulating leaf senescence.

Frontiers in Plant Science 13:860923.

Zhang Z, Liu C, Li K, Li X, Xu M, Guo Y* (2022) CLE14 functions as a “brake signal”

suppressing age-dependent and stress-induced leaf senescence through promoting JUB1-

mediated ROS scavenge in Arabidopsis. Molecular Plant 15:179-188.

Guo Y*, Hiatt E, Bonnet C, Kudithipudi D, Lewis RS, Shi H, Patra B, Zhao X, Dorlhac de

Borne F, Gilles T, Yang S, Zhang H, Zhang M, Lusso M, Berger IJ, Xu D, Wen L (2021)

Molecular regulation and genetic manipulation of alkaloid accumulation in tobacco plants.

Studies in Natural Products Chemistry (Atta-ur-Rahman edited), Elsevier Vol. 70:119-

149.

Guo Y^#, Ren G^#, Zhang K^#, Li Z^#, Miao Y*, Guo H* (2021) Leaf senescence: progression,

regulation, and application. Molecular Horticulture 1:5.

Wang Q^#, Guo C^#, Li Z, Sun J, Wang D, Xu L, Li X*, Guo Y* (2021) Identification and analysis

of bZIP family genes in potato and their potential roles in stress responses. Frontiers in

Plant Science 12:637343.

Li L^#, Li K^#, Ali A, Guo Y* (2021) AtWAKL10, a cell wall associated receptor-like kinase,

negatively regulates leaf senescence in Arabidopsis thaliana. International Journal of

Molecular Sciences 22(9): 4885.

Li W, Wen L, Chen Z, Zhang Z, Pang X, Deng Z, Liu T, Guo Y* (2021) Study on metabolic

variation in whole grains of four proso millet varieties reveals metabolites important for

antioxidant properties and quality traits. Food Chemistry 357: 129791.

Guo C^#, Wang Q^#, Li Z, Sun J, Zhang Z, Li X*, Guo Y* (2021) Bioinformatics and expression

analysis of IDA-Like genes reveal their potential functions in flower abscission and stress

response in tobacco (Nicotiana tabacum L.). Frontiers in Genetics 12: 670794.

Wang Q^#, Guo C^#, Li Z, Sun J, Deng Z, Wen L, Li X*, Guo Y* (2021) Potato NAC

transcription factor StNAC053 enhances salt and drought tolerance in transgenic Arabidopsis. International Journal of Molecular Sciences 22(5): 2568.

Li Z^#, Chao J^#, Li X, Li G, Song D, Guo Y, Wu X*, Liu G* (2021) Systematic analysis of the

bZIP family in tobacco and functional characterization of NtbZIP62 involvement in

salt stress. Agronomy 11: 148.

Zhang Z, Xu M and Guo Y* (2020) Ring/U-Box protein AtUSR1 functions in promoting leaf

senescence through JA signaling pathway in Arabidopsis. Frontiers in Plant Science 

11: 608589.

Zhang Z, Liu C and Guo Y* (2020) Wheat transcription factor TaSNAC11-4B positively

regulates leaf senescence through promoting ROS production in transgenic Arabidopsis.

International Journal of Molecular Sciences 21(20): 7672.

Yuan L, Wang D, Cao L, Yu N, Liu K, Guo Y, Gan S* and Chen L* (2020) Regulation of leaf

longevity by DML3-mediated DNA demethylation. Molecular Plant 13(8): 1149-1161.

Zhang Z, Li W, Gao X, Xu M and Guo Y* (2020) DEAR4, a member of DREB/CBF family,

positively regulates leaf senescence and response to multiple stressors in Arabidopsis

thaliana. Frontiers in Plant Science 11: 367.

Gao X^#, Wu X^#, Liu G, Zhang Z, Chao J, Li Z, Guo Y* and Sun Y* (2019) Characterization

and mapping of a novel premature leaf senescence mutant in common tobacco (Nicotiana

tabacum L.). Plants 8(10): 415.

Ahmad S and  Guo Y* (2019) Signal transduction in leaf senescence: progress and perspective.

Plants 8(10): 405.

Ren A^#, Ahmed RI^#, Chen H, Han L, Sun J, Ding A, Guo Y and Kong Y* (2019) Genome-wide

identification, characterization and expression patterns of the pectin methylesterase

inhibitor genes in Sorghum bicolor. Genes 10(10): 755.

Cao S, Zhang Z, Wang C, Li X, Guo C, Yang L and Guo Y* (2019) Identification of a novel

melon transcription factor CmNAC60 as a potential regulator of leaf senescence. Genes 

10(8): 584.

Li X^#, Guo C^#, Ahmad S, Wang Q, Yu J, Liu C and Guo Y* (2019) Systematic analysis of

MYB family genes in potato and their multiple roles in development and stress responses.

Biomolecules 9(8): 317.

Li X^#, Ahmad S^#, Ali A, Guo C, Li H, Yu J, Zhang Y, Gao, X, Guo Y* (2019) Characterization

of Somatic Embryogenesis Receptor-Like Kinase 4 as a negative regulator of leaf

senescence in Arabidopsis. Cells 8(1):50.

Li W, Li X, Chao J, Zhang Z, Wang W and Guo Y* (2018) NAC family transcription factors in

tobacco and their potential role in regulating leaf senescence. Frontiers in Plant Science 

9: 1900.

Li X^#, Ahmad S^#, Guo C, Yu J, Cao S, Gao X, Li W, Li H, Guo Y* (2018) Identification and

characterization of LRR-RLK family genes in potato reveal their involvement in peptide signaling of cell fate decisions and biotic/abiotic stress responses. Cells 7(9): 120.

Gao X, Guo Y* (2018) Research progress of leaf senescence related genes in tobacco. Journal

of Plant Sciences 6 (3): 87-92.

Li X^#, Hamyat M^#, Liu C, Ahmad S, Gao X, Guo C, Wang Y*, Guo Y* (2018) Identification

and characterization of the WOX family genes in five Solanaceae species reveal their

conserved roles in peptide signaling. Genes 9(5): 260.

Guo Y. (editor) (2018) Plant Senescence – Methods and Protocols, Humana Press, New

York, NY, 367 pages.

Li W, Guo Y* (2018) RNA-Seq analysis of the transcriptome of leaf senescence in tobacco.

Methods in Molecular Biology 1744: 331-337.

Zhang Z, Guo Y* (2018) Hormone treatments in studying leaf senescence. Methods in

  Molecular Biology 1744: 125-132.

Ali A, Gao X, Guo Y* (2018) Initiation, progression, and genetic manipulation of leaf

senescence. Methods in Molecular Biology 1744: 9-31.

Li W, Zhang H, Li X, Zhang F, Liu C, Du Y, Gao X, Zhang Z, Zhang X, Hou Z, Zhou H, Sheng

X, Wang G, Guo Y* (2017) Integrative metabolomic and transcriptomic analyses unveil

nutrient remobilization events in leaf senescence of tobacco. Scientific Reports 7(1):

12126.

Guo Y^#, Flegel K^#, Kumar J, McKay DJ, Buttitta LA* (2016) Ecdysone signaling induces two

phases of cell cycle exit in Drosophila cells. Biology Open, 5(11): 1648-1661.

Guo Y and Gan S* (2014) Translational researches on leaf senescence for enhancing plant

productivity and quality. Journal of Experimental Botany 65 (14): 3901-3913.

Li W and Guo Y* (2014) Transcriptome, transcription factors and transcriptional regulation of

leaf senescence. Journal of Bioinformatics and Comparative Genomics, 1: 1-3.

Guo Y (2013) Towards systems biological understanding of leaf senescence. Plant Molecular

Biology 82:519-528.

Gao X and Guo Y* (2012) CLE peptides in plants: proteolytic processing, structure-activity

relationship, and ligand-receptor interaction. Journal of Integrative Plant Biology

54(10): 738-45.

Guo Y and Gan S* (2012) Convergence and divergence in gene expression profiles induced by

leaf senescence and 27 senescence-promoting hormonal, pathological and environmental

stress treatments. Plant, Cell & Environment 35(3):644-655.

Guo Y, Ni J, Denver R, Wang X and Clark SE* (2011) Mechanisms of molecular mimicry of

plant CLE peptide ligands by the parasitic nematode Globodera rostochiensis. Plant

Physiology 157(1):476-484.

Guo Y and Gan S* (2011) AtMYB2 regulates whole plant senescence by inhibiting cytokinin-

mediated branching at late stages of development in Arabidopsis. Plant Physiology

156 (3): 1612-1619.

Ni J, Guo Y, Jin H, Hartsell J and Clark SE* (2011) Characterization of a CLE processing

 activity. Plant Molecular Biology 75(1-2): 67-75.

Guo Y, Han L, Hymes M, Denver RJ and Clark SE* (2010) CLAVATA2 forms a distinct

CLE-binding receptor complex regulating Arabidopsis stem cell specification. Plant

Journal 63(6): 889-900.

Guo Y and Clark SE* (2010) Membrane distributions of two ligand-binding receptor complexes

 in the CLAVATA pathway. Plant Signaling & Behavior 5(11): 1442-1445.

Zhou C, Cai Z, Guo Y and Gan S* (2009) An Arabidopsis Mitogen-Activated Protein Kinase

Cascade, MKK9-MPK6, Plays a Role in Leaf Senescence. Plant Physiology 150: 167-77.

Stenvik GE, Tandstad NM, Guo Y, Shi CL, Kristiansen W, Holmgren A, Clark SE, Aalen RB

and Butenko MA* (2008) The EPIP peptide of INFLORESCENCE DEFICIENT IN

ABSCISSION is sufficient to induce abscission in arabidopsis through the receptor-like

kinases HAESA and HAESA-LIKE2. Plant Cell 20(7): 1805-17.

Guo Y and Gan S* (2007) Genetic manipulation of leaf senescence. In “Senescence Processes

in Plants” (S. Gan, ed.), Blackwell Publishing, Oxford, UK, pp. 304-322.

Guo Y and Gan S* (2006) AtNAP, a NAC family transcription factor, has an important role in

leaf senescence. Plant Journal 46: 601-612.

Guo Y and Gan S* (2005) Leaf senescence: signals, execution, and regulation. Current Topics

in Developmental Biology 71: 83-112.

Guo Y^#, Cai Z^# and Gan S* (2004) Transcriptome of Arabidopsis leaf senescence. Plant,

Cell & Environment 27: 521-549. (Featured in: Gepstein S. (2004) Leaf senescence--not

just a 'wear and tear' phenomenon. Genome Biol. 5(3):212) ^# Co-first authors.